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Maize genome sequence and gene model files (version 4.32) were download from ensemble plants ( ). The sRNA and mRNA sequencing data of different maize genotypes have been deposited in National Genomics Data Center ( ) with a GSA accession number CRA003871, which will be reached via the link. These results indicate that TE-IR-mediated posttranscriptional regulation is a key molecular mechanism underlying the tradeoff between crop environmental adaptation and yield-related traits, providing potential genomic targets for the breeding of crops with greater stress tolerance but uncompromised yield. A genome-wide scan revealed that TE-IRs associate with drought response and yield-related traits that were positively selected and expanded during maize domestication. Transgenic and molecular studies of Drought-Related Environment-specific Super eQTL Hotspot on chromosome 8 ( DRESH8) and ZmMYBR38, a target of DRESH8-derived small interfering RNAs, revealed a transposable element-mediated inverted repeats (TE-IR)-derived sRNA- and gene-regulatory network that balances plant drought tolerance with yield-related traits. Here we carried out genome-wide profiling of the small RNA (sRNA) transcriptome (sRNAome) and transcriptome landscapes of a global maize diversity panel under dry and wet conditions and uncover dozens of environment-specific regulatory hotspots.

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The genomic basis underlying the selection for environmental adaptation and yield-related traits in maize remains poorly understood.











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